O-157 Modelling human early development

Abstract Understanding human peri-implantation development is necessary to improve in vitro fertilisation. Technical progress, such as scRNAseq and integrated stem cell models have dramatically improved our ability understand development. However, in-depth analysis systematic validation embryos remains build a reliable reference. Here, we started by integrating pre- post-implantation embryo datasets generate reference, highlighting progression of fates during early Second, identified the dynamics genes modules, groups with similar expression pattern. In particular, this highlighted hierarchy transcription factor potentially driving fate progression. Importantly, performed prolonged culture validate immunofluorescence EPI TE fates. Moreover, compared profile markers models. Finally, used combination gene modules benchmark blastoid Altogether, study paves way for modelling Trial registration number NA Study funding: Funding source: